The WISP1 Polyclonal Antibody (CAB3346) is a valuable tool for researchers studying WISP1, a protein involved in cell growth, inflammation, and tissue repair. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the WISP1 protein, this antibody allows for the detection and analysis of WISP1 in various cell types, making it an essential asset in studies related to immunology, cancer, and tissue regeneration.WISP1, also known as CCN4, is known for its role in promoting cell proliferation, migration, and survival in various tissues. Its overexpression has been linked to tumor growth and metastasis, making it a potential target for cancer therapy.
Additionally, WISP1 has been implicated in promoting tissue repair and fibrosis, suggesting its potential relevance in regenerative medicine and wound healing research.By offering a specific tool for investigating the function and regulation of WISP1, the CAB3346 antibody enables researchers to deepen their understanding of the roles this protein plays in both physiological and pathological processes. Its versatility and reliability make it an essential component of any lab focused on elucidating the complex functions of WISP1 in health and disease.
Product Name:
WISP1 Rabbit Polyclonal Antibody
SKU:
CAB3346
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 158-367 of human WISP1 (NP_003873.1).
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
Purification Method:
Affinity purification
Gene ID:
8840
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using WISP1 antibody (CAB3346) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
