Cell-Based ELISA Kits

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Assay Genie Cell-Based ELISA Kits

Forget about Western Blotting with the Assay Genie Cell-Based ELISA Kits! These convenient, lysate-free 96-well ELISA can determine target protein levels via an indirect, in vitro immunohistochemistry based technique. This is an ideal technology to screen for the effects of inhibitors & activators on protein expression in cell culture directly without the need to make cell lysates.

Features

  • Throughput High throughput screening using 96-well ELISA technology
  • Rapid Screen in situ with no lysate preparation required
  • Convenient Assess the effects of activators and inhibitors on cellular processes
  • Adaptable Analyse proteins in adherent and suspension cells

Key Assay Genie Cell-Based ELISA Kits

14-3-3 beta/zeta ERK3 NMDAR1
14-3-3 zeta ERK8 NMDAR2B
4E-BP1 ES8L1 nNOS
53BP1 Estrogen Receptor-alpha NPM
60S Ribosomal Protein L10 Estrogen Receptor-beta NR1I3
AAK1 ETK NR2F6
ABHD6 ETS1 Nrf2
ABHD7 ETV6 NRG1 Isoform-10
ABHD9 Ezrin Nuclear Factor 1
ABL1 FADD Nuclear Receptor NR4A1
ACC1 FAK NudC
ACK1 FAKD1 NYREN18
Actin- alpha-1 FAKD3 Oct1
Actin-gamma2 FAM84B Oct2
Actinin alpha-2/3 FANCD2 Oct3
Actin-pan FAP-1 OGR-1
ACTL6A FAS Opioid Receptor
ACV1B FAS ligand Opioid Receptor-delta
ACVL1 FER Osteopontin
ADA2L FES OVOL1
ADARB1 FGFR1 OXSR1
ADCK1 FGFR1 Oncogene Partner p16 INK
ADCK2 FGFR2 p21 Cip1
ADCK4 FGFR3 p27 Kip1
ADCK5 FHIT p300
ADD1/2 Filamin A p300/CBP
ADD2 FIR p38 MAPK
ADRB2 FKHR p42 MAPK
Adrenergic Receptor alpha-2C FKHRL1 p44/42 MAP Kinase
Adrenergic Receptor beta2 FLI1 p47 phox
AF10 FLT3 p50 Dynamitin
AFX Fos p53
AhR FosB p56 Dok-2
AIRE FOXB1/2 p57 Kip2
AKAP1 FOXE3 p62 Dok
AKAP10 FOXJ1 p63
AKAP13 FOXK1 p70 S6 Kinase
AKAP14 FOXL1 p70 S6 Kinase beta
AKAP5 FOXN2 p73
AKAP8 FOXO1A p90 RSK
Akt1 S1 FOXO4 p95-NBS1
AKT1/2/3 Fra-2 PAK1
AKT1/3 FRK PAK2
Akt2 FRS2 PAK3
ALDOA Fyn Parathyroid Hormone
ALK G3BP-1 Parkin
ALOX5 Gab2 Patched
ALX3 GABA-B Receptor Pax-5
AML1 GAD1 Paxillin
AMPK beta1 GAD1/2 PBK/TOPK
AMPK1 GADD153 PDCD4
Amylin Galectin 3 PDE4D
Androgen Receptor GALK1 PDGFB
ANKK1 GANP PDGFRalpha
ANKRD1 GAS41 PDGFRbeta
Annexin A6 Gastrin PDK1
AOS1 GATA1 PDLIM1
AP-2 GATA4 PEA-15
APAF-1-ALT GATA6 PEK/PERK
APC GIRK1/KIR3.1/KCNJ3 Peripherin
APEX1 GIT2 PFDN1
APLP2 GK2 PFKFB1/4
A-RAF GK3 PFKFB2
ARBK1 GLU2B PGBD1
ARC Glucagon PGP9.5
ARHGEF2 Glucokinase Regulator PHCA
ARMCX2 GluR1 PI3-kinase p85-alpha/gamma
ARNT GluR2 PIAS1
Arrestin 1 GluR4 PIAS3
ASK1 GluR5 PIAS4
ATF1 GLUT1 Pim-1
ATF2 GLUT3 PIP5K
ATF3 Glycogen Synthase PITX1
ATF5 GR PKA-R2beta
ATF7 Granzyme B PKC alpha
ATP1A1 GRB10 PKC delta
ATPase GRK1 PKC epsilon
ATP-Citrate Lyase GRK2 PKC theta
ATRIP GRK3 PKC zeta
AurA GRK5 PKCB
AurB GRK7 PKD1/2/3
AurB/C GSC2 PKD1/PKC mu
BACH1 GSK3alpha PKD2
BAD GSK3alpha/beta PKR
Bak GSK3beta PLCbeta3
BAX Guanylate Cyclase beta PLCG1
BCAR1 HAT PLCG2
BCAR3 HBP1 PLCH
BCL-2 hCG beta PLK1
Bcl-6 HDAC1 PML
BCL-XL HDAC10 PMP22
Bcr HDAC2 POLR2A
BID HDAC3 Potassium Channel Kv3.2b
BIK HDAC4 PP2A-alpha
BIM HDAC5 PPAR-gamma
BL-CAM HDAC6 PPIF
BLNK HDAC7 PPP1R14C
BMX HDAC8 PPP1R2
B-Myb HDAC9 Presenilin 1
B-RAF HER2 PRKAG1/2/3
BRCA1 HER3 Progesterone Receptor
BRD3 HEXB Prostate Apoptosis Response protein-4
Breast Tumor Kinase Hexokinase-3 Prostate-specific Antigen
BRSK1 HGS Proteasome alpha3
BTK HINT1 PSEN1
BUD31 Histone H1 PSMD2
C/EBP-alpha Histone H2A.X PTEN
C/EBP-beta Histone H2B PTP1B
C/EBP-epsilon Histone H3 PTTG
Cadherin-pan Histone H4 PTTG1
Calcium Sensing Receptor HKR1 PYK2
Caldesmon HNF4 alpha/gamma QORX
Calmodulin HNF4alpha Rabphilin 3A
Calnexin hnRNP C1/C2 RAD17
CALR hnRNP H RAD51
Calsenilin/KCNIP3 hnRNP K RAD52
CaMK1-alpha hnRNP M RASH/RASK/RASN
CaMK1-beta hnRNP Q RBM26
CAMK2A/CAMK2D HORMAD1 RBM5
CaMK2-beta/gamma/delta HOXA1 RCBTB1
CaMK4 HOXA7 Recoverin
CARD6 HOXB9 Rel
Casein Kinase II alpha HOXD12 RelB
Caspase 1 HP1gamma REQU
Caspase 10 HRH1 Ret
Caspase 2 HS1 Retinoblastoma
Caspase 3 HSF1 Retinoic Acid Receptor beta
Caspase 6 HSF2 Retinoid X Receptor gamma
Caspase 8 HSL RFA2
Caspase 9 HSP10 RFWD2
Catenin-alpha1 HSP27 RGAG1
Catenin-beta HSP40 Rhodopsin
Catenin-delta1 HSP90B RhoH
Catenin-gamma ICAM-1 RIPK2
Caveolin-1 ICK RIT1
CBL ID4 RPS6KC1
CBP IGF1R RSK1/2/3/4
CBX5 IGFBP-3 S1P Receptor EDG1
CBX6 IkappaB-alpha SDCG1
CCR5 IkappaB-beta SEK1/MKK4
CCRK IkappaB-epsilon SENP1
CD147 IKK-alpha SENP2
CD19 IKK-alpha/beta SENP5
CD226 IKK-beta SENP7
CD253 IKK-gamma SENP8
CD3 zeta IL-10Ralpha SEPARASE
CD32 IL-13R/CD213alpha1 Sept1
CD40 IL-2Ralpha SERC3
CD5 IL-2Rbeta SF1
CD50/ICAM-3 IL-3Rbeta SFRS4
CD71/TfR IL-4R/CD124 SGK
CD88/C5aR ILK SGOL1
CDC2 iNOS Shc
CDC25A Integrin beta1 SHIP1
CDC2L1 Integrin beta3 SHP-1
CDC6 Integrin beta4 SHP-2
CDK1/CDC2 Interferon-gamma Receptor alpha SIAH1
CDK10 IP3KA Sirp alpha1
CDK2 IP3KC SIX6
CDK5 IPMK SKP1A/p19
CDK5R1 IR SKP2/p45
CDK5R2 IRF2 Smad1
CDK7 IRF3 Smad1/5/9
CDK8 IRF4 Smad2
CDKL1 IRS-1 Smad2/3
CDKL4 IRX1 Smad3
CDKN3 ISL2 Smad4
CEBPB ITCH SMC1
CEBPD/E ITPK1 SMG7
CEBPZ JAB1 SMRC2
CERKL JAK2 SNAI1
CGK 2 JAK3 SNAP25
Chk1 JIP2 Sodium Channel-pan
Chk2 JM4 SOX6
c-Jun JMY SOX8/9/17/18
CKI-alpha JNK1/2/3 SOX9
CKI-epsilon JunD SP1
CKI-gamma2 K6PP SP2
CKII-beta KAP0 SPHK2
CKMT2 KAP1 SPI1
CKS1 KAPC A/B Src
Claudin 1 KAPCB SREBP-1
Claudin 10 Keratin 10 SRF
Claudin 11 Keratin 14 SRY
Claudin 2 Keratin 15 SSB
Claudin 3 Keratin 16 SSBP2
Claudin 4 Keratin 17 SSXT
Claudin 5 Keratin 18 ST5
Claudin 6 Keratin 19 STAT1
Claudin 7 Keratin 20 STAT2
CLK2 Keratin 5 STAT3
Clock Keratin 8 STAT4
CNOT2 KIF11/Eg5 STAT5A
Coagulation Factor III KIF20A STAT5A/B
Cofilin Kir6.2 Stathmin 1
Collagen I alpha2 KLF11 STEA3
Collagen IV KNTC2 Stefin A
Collagen IV alpha2 KPBB Stefin B
Collagen V alpha1 KRT37/38 STK39
Collagen VI alpha2 KSR Sumo1
Collagen XI alpha1 KSR2 SURF1
Collagen XX alpha1 Ku70/80 Survivin
Connexin 43 Ku70/XRCC6 SYK
COPS2 Ku80/XRCC5 Synapsin1
COPS5 Lamin A/C Synaptophysin
Cortactin LAT Synaptotagmin
Cox1 LAT3 Synuclein
CPI17alpha Lck Synuclein-pan
c-PLA2 LDOC1L TACC1
C-RAF LHR2A TAF1A
CREB LIMK1 TAF4
CREB-BP LIMK1/2 TAF5L
CREM LKB1 TAL-1
CrkII LMO3 TALL-2
CrkL LMTK2 Tau
CRMP2 LMX1B TBC1D3
CRP1 LTK TBX1
CSE1L Lyn TBX10
CSFR M3K13 TBX15/18
CSK MADD TBX2
CSTF1 MAEA TCOF1
CtBP1 Maf TEBP
Cullin 1 MAGE-1 TEF
Cullin 2 MAK Telomerase
Cyclin A Mammaglobin TF2A1
Cyclin A1 Mammaglobin B TF2H2
Cyclin B1 MAP2K6 TF3B
Cyclin D1 MAP2K7 TF3C2
Cyclin D2 MAP3K7 TGF alpha
Cyclin D3 MAP3K8 TGF beta Receptor II
Cyclin E1 MAP3KL4 TGF beta1
Cyclin E2 MAP4K6 TGF beta3
Cyclin G MAPK3 THRB
Cyclin H MAPKAPK2 TIE2
Cyclin L1 MAPKAPK5 TIF-IA
Cytochrome c MARCKS TIMP2
Cytochrome P450 17A1 MARK3 TIMP3
Cytochrome P450 21A2 MART-1 TIMP4
Cytochrome P450 24A1 MAST3 TIP60
Cytochrome P450 26A1 MAST4 TISB
Cytochrome P450 27A1 MASTL TISD
Cytochrome P450 2B6 MAT1 TLE4
Cytochrome P450 2C19 MBD3 TMPRSS3
Cytochrome P450 2C8/9/18/19 MCL1 TNF Receptor II
Cytochrome P450 2D6 MCM2 TNF12
Cytochrome P450 2J2 MCM4 TNFC
Cytochrome P450 2R1 MCM5 TNFRSF9
Cytochrome P450 2S1 MDM2 TNFSF11
Cytochrome P450 2U1 MDM4 TNFSF15
Cytochrome P450 39A1 MED1 TNFSF9
Cytochrome P450 3A4/5 MED26 TNNI3
Cytochrome P450 3A43 MEF2A TNR16
Cytochrome P450 3A7 MEF2B TOB1
Cytochrome P450 4B1 MEK1 TOP2A
Cytochrome P450 7B1 MEK1/2 TOP2B
Dab1 MEK2 TP53I11
DAK MELK TPH2
DAPK3 MEOX2 TRA-2 alpha
DAPP1 Merlin TRADD
DARPP-32 Met Transglutaminase 2
Daxx Mevalonate Kinase TRI18
DCLK2 mGluR2/3 TRIM24
DDR1 mGluR4 TRIM3
DDX3 mGluR6 TRIM38
Dematin mGluR7 TRIM59
Desmin mGluR8 TRIP13
DGKA MITF Trk A
DGKD MKK3 Trk B
DGKE MKK6 TRXR2
DGKI MKP1 Tryptophan Hydroxylase
DGKQ MKP-1/2 TSC22D1
DJ-1 MMP-1 TSN
DLGP1 MMP-11 TSPAN8
DLX3 MMP-13 TTC23
DLX4 MMP-14 Tuberin/TSC2
DLX5 MMP-15 Tubulin alpha
DMPK MMP-16 Tubulin beta
DNA Polymerase alpha MMP-19 Tubulin gamma
DNA Polymerase beta MMP-2 TUSC2
DNA-PK MMP-23 TUSC3
DOK7 MMP-3 Tyrosinase
Doublecortin MMP-7 Uba2
DP-1 MMP-8 UBE1L
DUSP4 MMP-9 UBF1
Dynamin-1 MN1 USF2
DYR1A MNK1 VASP
Dysferlin MOBKL2B VAV1
Dyskerin Moesin/Ezrin/Radixin VAV2
E2F1 MOL2C VAV3
E-cadherin MRE11 VCP
EDD MSK1 VE-Cadherin
eEF2 MSK2 VEGFB
eEF2K Mst1/2 VEGFR2
EFNA3 MTA1 Vimentin
EFNA4 Mtor Vitamin D Receptor
EFNA5 MtSSB VPS72
EFNB1 Musculin WASP
EFNB3 MYB WAVE1
EGFR MYC WEE1
EGR1/2 Myelodysplasia Syndrome 1 WTAP
eIF2alpha MYF6 WWOX
eIF4B Myogenic Factor 5 XIAP
ELAVL2 MYPT1 XPA
ELF1 MYT1 XPF
Elk1 NAB2 XRCC1
ELL NBL1 XRCC4
eNOS NCBP1 XRCC6
EPB41 NCBP2 YAP
EPHA1 NCoR1 ZEB2
EPHA2/3/4 NDF2 ZIC1/2/3
EPHA5 NEDD8 ZNF134
EPHA7 NEK9 ZNF147
EPHB1/2 NFAT3 ZNF174
EPHB1/2/3/4 NFAT4 ZNF187
EPHB2 NFIL-3 ZNF24
EPHB6 NF-kappaB p100/p52 ZNF265
Epo-R NF-kappaB p105/p50 ZNF397
ER81 NF-kappaB p65 ZNF596
ERAB NFYB ZNF638
ERCC1 NKX6.3 ZNF668
ERCC5 NM23 ZNF785

Cell-Based ELISA Overview


Contents

Fluorometric Cell-Based ELISA Kits

  • TBS
  • Quenching Buffer
  • Blocking Buffer
  • Wash Buffer
  • Primary Antibody Diluent
  • α-Target Antibody
  • α-GAPDH Antibody
  • Dye-1 Conjugated Anti-Rabbit IgG Antibody
  • Dye-2 Conjugated Anti-Mouse IgG Antibody
  • 96-Well Black Cell Culture Clear-Bottom Microplate
  • Adhesive Plate Seals

Colorimetric Cell-Based ELISA Kits

  • TBS
  • Quenching Buffer
  • Blocking Buffer
  • Wash Buffer
  • Primary Antibody Diluent
  • α-Target Antibody
  • α-GAPDH Antibody
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • SDS Solution
  • Crystal Violet Solution
  • Ready-to-use Substrate
  • Stop Solution
  • 96-Well Cell Culture Clear-Bottom Microplate
  • Adhesive Plate Seals

Cell-Based ELISA Princple

Fluorometric Cell-Based ELISA

For Fluorometric Cell-Based ELISA, the target protein is captured by target-specific primary (1°) antibodies while Dye 1-conjugated and Dye 2-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the dye conjugated to the 2° antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method.

A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. If a phosphorylated target is being detected, an antibody against the nonphosphorylated counterpart will be provided for normalization purposes.

Figure : Assay format of Assay Genie Fluorometric Cell-Based ELISA Kit


Figure : Assay format of Assay Genie Colorimetric Cell-Based ELISA Kit

Colorimetric Cell-Based ELISA

For Colorimetric Cell-Based ELISA, the target protein is captured by target-specific primary (1°) antibodies while the HRP-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the HRP enzyme conjugated to the 2° antibody can catalyze a colorimetric reaction upon substrate addition.


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