The UCP1 Monoclonal Antibody is a powerful tool for researchers studying UCP1, a protein involved in thermogenesis and energy expenditure in brown adipose tissue. This monoclonal antibody, produced using hybridoma technology, is highly specific to UCP1 and has been validated for use in various applications, including immunohistochemistry and flow cytometry.UCP1, also known as the uncoupling protein 1, is a key regulator of thermogenesis by allowing the dissipation of energy as heat. This unique function makes UCP1 a promising target for research into metabolic disorders, obesity, and diabetes. By using the UCP1 Monoclonal Antibody, researchers can accurately detect and quantify UCP1 expression in different cell types and tissues, aiding in the understanding of its role in energy metabolism and potential therapeutic interventions.
This antibody is an essential tool for researchers interested in unraveling the complex mechanisms of energy regulation and metabolic health, offering reliable and reproducible results for a wide range of experimental settings. By targeting UCP1 with precision, this antibody opens up new possibilities for investigating the role of brown adipose tissue in energy balance and its implications for human health.
Product Name:
UCP1 Monoclonal Antibody
SKU:
CAB21979
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human UCP1 (NP_068605.1).
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.
Purification Method:
Affinity purification
Gene ID:
7350
Clone Number:
ARC54141
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using UCP1 antibody (CAB21979) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
