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Anti-Tri-methyl-Histone H3(K4) Antibody (MACO0074)

SKU:
MACO0074
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Mouse
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Monoclonal Antibody
Conjugation:
Unconjugated
€269
Frequently bought together:

Description

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Anti-Tri-methyl-Histone H3(K4) Antibody (MACO0074)

The Tri-Methyl Histone H3K4 Monoclonal Antibody (MAC0074) is a powerful tool for researchers studying epigenetics and chromatin remodeling. This antibody is specifically designed to target the tri-methylated form of histone H3 at lysine 4, a modification associated with active gene transcription.Raised in mice, this monoclonal antibody is highly specific and shows strong reactivity with the tri-methyl histone H3K4 modification in a variety of species, including human, mouse, and rat samples. It has been rigorously validated for use in techniques such as Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence, allowing for precise detection and visualization of tri-methylated histone H3K4 in cell and tissue samples.

By targeting this specific histone modification, researchers can gain insights into gene regulation, chromatin structure, and cellular differentiation processes. The Tri-Methyl Histone H3K4 Monoclonal Antibody provides a valuable tool for studying epigenetic mechanisms underlying development, disease, and potential therapeutic interventions.

Product Name:Anti-Tri-methyl-Histone H3(K4) Antibody
Product Sku:MACO0074
Size:50ug
Host Species:Mouse
Tested Applications:ELISA, WB
Recommended Dilutions:WB:1:500-1:5000
Species Reactivity:Human,Mouse,Rat
Immunogen:Synthetic Peptide
Form:Liquid
Storage Buffer:PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Purification Method:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clonality:Monoclonal
Isotype:IgG
Conjugate:Non-conjugated
Background:
Synonyms:HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/
Product ImageWestern blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7, diluted at 1:1000.
UniProt Protein Function:H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex

Molecular Function:cadherin binding; histone binding; protein binding

Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P68431
NCBI GenInfo Identifier:55977055
NCBI Gene ID:8357
NCBI Accession:P68431.2
UniProt Secondary Accession:P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1,
UniProt Related Accession:P68431
Molecular Weight:15kDa
NCBI Full Name:Histone H3.1
NCBI Synonym Full Names:histone cluster 1 H3 family member h
NCBI Official Symbol:HIST1H3H  
NCBI Official Synonym Symbols:H3/k; H3FK; H3F1K  
NCBI Protein Information:histone H3.1
UniProt Protein Name:Histone H3.1
UniProt Synonym Protein Names:Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name:HIST1H3A  

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