The TCEB2 Polyclonal Antibody (CAB13363) is a valuable tool for researchers studying TCEB2, a key protein involved in the regulation of transcription and protein degradation. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the TCEB2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an invaluable asset for studies in molecular biology and cancer research.TCEB2, also known as transcription elongation factor B, is essential for promoting the elongation of RNA polymerase II during transcription. Its role in regulating gene expression makes it a target of interest in understanding various biological processes, including cell growth, differentiation, and disease development.
By investigating the function of TCEB2, researchers can gain insights into the mechanisms underlying these processes and potentially identify new therapeutic targets for diseases such as cancer and genetic disorders.Overall, the TCEB2 Polyclonal Antibody (CAB13363) offers researchers a reliable tool for studying the function and regulation of TCEB2 in various biological contexts. Its high specificity and sensitivity make it a valuable asset for advancing our understanding of transcriptional regulation and its implications for human health.
Product Name:
TCEB2 Rabbit Polyclonal Antibody
SKU:
CAB13363
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human TCEB2 (NP_009039.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
SIII; TCEB2
Positive Sample:
22Rv1,NCI-H460,MCF7
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
13kDa
Observed MW:
16kDa
This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.
Purification Method:
Affinity purification
Gene ID:
6923
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TCEB2 Rabbit pAb (CAB13363) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
