The TAF5L Polyclonal Antibody (PAC043850) is a valuable tool for researchers studying the TAF5L protein, a critical component of the TFIID complex involved in transcription initiation. This antibody is produced in rabbits and is highly specific for human samples, making it ideal for Western blot applications. By binding to TAF5L, this antibody enables researchers to detect and analyze the protein in various cell types, facilitating research in the fields of molecular biology and gene regulation.TAF5L is essential for the assembly and stability of the TFIID complex, which is responsible for recruiting RNA polymerase II to gene promoters and initiating transcription.
Dysregulation of TAF5L expression has been linked to various diseases, including cancer and developmental disorders. Therefore, studying the function of TAF5L is critical for gaining insights into the molecular mechanisms underlying these conditions and identifying potential therapeutic targets.By using the TAF5L Polyclonal Antibody, researchers can enhance their understanding of TAF5L's role in transcriptional regulation and its involvement in disease pathology. This antibody provides a reliable tool for investigating TAF5L function and opens up new opportunities for research in genetics, molecular biology, and personalized medicine.
Antibody Name:
TAF5L Antibody (PACO43850)
Antibody SKU:
PACO43850
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L protein (1-325AA)
Form:
Liquid
Storage Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method:
Antigen Affinity Purified
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot. All lanes: TAF5L antibody at 10µg/ml + Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 67, 37 kDa. Observed band size: 67 kDa.
Background:
Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex.
TAF5L: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Belongs to the WD repeat TAF5 family. 2 isoforms of the human protein are produced by alternative splicing.
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
NCBI Summary:
The product of this gene belongs to the WD-repeat TAF5 family of proteins. This gene encodes a protein that is a component of the PCAF histone acetylase complex. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. The encoded protein is structurally similar to one of the histone-like TAFs, TAF5. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
