The TAF1A Polyclonal Antibody (PACO21858) is a valuable tool for researchers studying TAF1A, a key component of the TFIID complex involved in transcriptional regulation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the TAF1A protein, this antibody allows for the detection and analysis of TAF1A in a variety of cell types, making it an essential reagent for studies in molecular biology and gene expression.TAF1A, also known as TAF1 RNA polymerase II, is essential for the initiation of transcription and plays a crucial role in the regulation of gene expression.
Dysfunction of TAF1A has been implicated in various diseases, including cancer and developmental disorders, making it an attractive target for research in these areas. By studying the function of TAF1A, researchers can gain insights into the mechanisms of transcriptional control and potentially identify novel therapeutic strategies for treating related diseases.
Antibody Name:
TAF1A Antibody (PACO21858)
Antibody SKU:
PACO21858
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Rat
Immunogen:
Synthesized peptide derived from internal of human TAF1A.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Gregory S.G., Nature 441:315-321(2006).
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, using TAF1A antibody.
TAF1A: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. 2 isoforms of the human protein are produced by alternative splicing.
Biological Process: positive regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter
NCBI Summary:
This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq, Jan 2011]
