The SP3/SP4 Polyclonal Antibody (PACO21821) is a valuable tool for researchers studying SP3 and SP4 proteins, key transcription factors involved in gene regulation. This rabbit-raised antibody shows high reactivity with human samples and is validated for Western blot applications, allowing for the detection and analysis of SP3/SP4 proteins in a variety of cell types.SP3 and SP4 are members of the Sp/KLF transcription factor family and are known to play important roles in the regulation of gene expression, cell proliferation, and differentiation. Dysregulation of SP3/SP4 has been implicated in various diseases, including cancer and developmental disorders.
Understanding the function and regulation of these transcription factors is essential for advancing our knowledge of gene expression and potentially identifying novel therapeutic targets.Researchers in the fields of molecular biology, genetics, and biochemistry will find the SP3/SP4 Polyclonal Antibody (PACO21821) to be a reliable tool for their experimental studies aimed at unraveling the intricate mechanisms of gene regulation and cellular processes. Whether investigating basic research questions or exploring therapeutic avenues, this antibody is a valuable asset for unlocking the secrets of SP3 and SP4 proteins.
Antibody Name:
SP3/SP4 Antibody (PACO21821)
Antibody SKU:
PACO21821
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from C-terminal of human SP3/4.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Ota T., Nat. Genet. 36:40-45(2004).
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells, using SP3/4 antibody.
Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
NCBI Summary:
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]
