The SOX2 Polyclonal Antibody (CAB0561) is a valuable tool for researchers studying the SOX2 protein, a transcription factor crucial for maintaining pluripotency in stem cells and regulating development in various tissues. This antibody, specifically raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.SOX2 plays a pivotal role in stem cell maintenance, differentiation, and reprogramming, making it a key player in regenerative medicine and cancer research. By binding to the SOX2 protein, this antibody allows for precise detection and analysis in a variety of cell types, providing researchers with a reliable tool for investigating the function and expression of SOX2 in various biological contexts.
Understanding the function and regulation of SOX2 is essential for advancing our knowledge of developmental biology, tissue regeneration, and disease pathogenesis. The SOX2 Polyclonal Antibody (CAB0561) offers researchers a powerful tool to explore the intricate mechanisms governed by the SOX2 transcription factor, paving the way for innovative discoveries in the fields of stem cell biology, regenerative medicine, and cancer research.
Product Name:
[KO Validated] SOX2 Rabbit Polyclonal Antibody
SKU:
CAB0561
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ANOP3; MCOPS3; SOX2
Positive Sample:
293T,MCF7,Mouse lung,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
35kDa
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Purification Method:
Affinity purification
Gene ID:
6657
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using SOX2 Rabbit pAb (CAB0561) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
