The SMC1A Polyclonal Antibody (CAB7008) is a highly specific antibody designed for research involving SMC1A, a protein critical for maintaining genome stability and regulating DNA repair processes. This antibody, produced in rabbits, has been validated for use in Western blot applications and shows high reactivity with human samples.SMC1A, a subunit of the cohesin complex, plays a crucial role in ensuring the faithful segregation of chromosomes during cell division. Dysregulation of SMC1A function has been linked to various diseases, including cancer and developmental disorders.
Therefore, studying the expression and function of SMC1A is essential for understanding its role in these disease processes.By detecting and analyzing SMC1A protein levels in different cell types, the SMC1A Polyclonal Antibody can provide valuable insights into the molecular mechanisms underlying DNA repair and genomic stability. This makes it an ideal tool for researchers in the fields of genetics, molecular biology, and cancer research who are interested in exploring the roles of SMC1A in disease development and progression.
Product Name:
SMC1 Rabbit Polyclonal Antibody
SKU:
CAB7008
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SMC1 (NP_006297.2).
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
8243
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SMC1 antibody (CAB7008) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
