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Smad6 Polyclonal Antibody (CAB24035)

SKU:
CAB24035
€129
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Smad6 Polyclonal Antibody
SKU:CAB24035
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 231-330 of human Smad6 (NP_005576.3).
Sequence:RWPD LQHA VELK PLCG CHSF AAAA DGPT VCCN PYHF SRLC GPES PPPP YSRL SPRD EYKP LDLS DSTL SYTE TEAT NSLI TAPG EFSD ASMS PDAT KPSH
Tested Applications:WB
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:AOVD2; MADH6; MADH7; HsT17432; Smad6
Positive Sample:HeLa,A549
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:53kDa
Observed MW:70kDa

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:4091
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Smad6 knockdown (KD) HeLa cells using [KD Validated]Smad6 Rabbit pAb (CAB24035) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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