The SMAD2 Monoclonal Antibody is a valuable tool for researchers studying the SMAD2 protein, which is a critical mediator of the TGF-beta signaling pathway. This antibody, produced through monoclonal antibody technology, is specifically designed to target and detect SMAD2 in various research applications.SMAD2 is a key player in cellular processes such as growth, differentiation, and apoptosis, making it an essential protein to study in the fields of cell biology, developmental biology, and cancer research. The SMAD2 Monoclonal Antibody is highly specific for human samples and has been validated for use in techniques such as Western blotting and immunofluorescence.
By using the SMAD2 Monoclonal Antibody, researchers can gain valuable insights into the function and regulation of SMAD2 in normal physiological processes and disease states. This antibody's high reactivity and specificity make it a reliable tool for accurately detecting and analyzing SMAD2 expression in a variety of cell types, providing researchers with the means to advance their understanding of TGF-beta signaling and its implications in health and disease.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Clone Number:
ARC57635
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using [KO Validated] Smad2 Rabbit mAb (CAB22635) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
