The Siglec12 Polyclonal Antibody (CAB8519) is a valuable tool for researchers studying Siglec12, a member of the sialic acid-binding immunoglobulin-like lectin family. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the Siglec12 protein, this antibody allows for the detection and analysis of Siglec12 expression in various cell types, making it ideal for immunology and cancer research studies.Siglec12 is known to play a role in immune regulation, with implications in modulating immune responses.
Its involvement in immune system function makes it a promising target for research related to cancer, autoimmune diseases, and chronic inflammatory conditions. By gaining a better understanding of Siglec12 activity, researchers can develop new therapies that harness or regulate immune responses in these disease contexts. The Siglec12 Polyclonal Antibody (CAB8519) provides a reliable means to investigate the role of Siglec12 in immune regulation and its potential as a therapeutic target.
Product Name:
SIGLEC12 Rabbit Polyclonal Antibody
SKU:
CAB8519
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLEC12 (NP_443729.1).
Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
89858
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SIGLEC12 antibody (CAB8519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
