The Shank2 Polyclonal Antibody (CAB18423) is a valuable tool for researchers studying Shank2, a scaffolding protein involved in synaptic function and neuronal signaling. This rabbit polyclonal antibody is highly specific and reactive with Shank2 in human samples, making it ideal for use in Western blotting and immunohistochemistry applications.Shank2 plays a crucial role in organizing and stabilizing the postsynaptic density of excitatory synapses, impacting synaptic transmission and plasticity. Dysregulation of Shank2 has been linked to various neurological disorders, including autism spectrum disorders and intellectual disabilities.
Therefore, studying the expression and function of Shank2 using this antibody can provide insights into the molecular mechanisms underlying these conditions.By targeting Shank2, researchers can investigate its interactions with other synaptic proteins, its role in neuronal development and plasticity, and its potential involvement in neurodevelopmental disorders. This antibody offers the specificity and sensitivity needed to accurately detect and analyze Shank2 in various experimental settings, advancing our understanding of synaptic function and neurological disorders.
Product Name:
SHANK2 Rabbit Polyclonal Antibody
SKU:
CAB18423
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human SHANK2 (NP_036441.2).
apical plasma membrane, ciliary membrane, cytosol, dendritic spine, glutamatergic synapse, growth cone, hippocampal mossy fiber to CA3 synapse, neuron projection, plasma membrane, postsynaptic density, presynaptic active zone
Calculated MW:
201kDa
Observed MW:
159kDa
This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
22941
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SHANK2 Rabbit pAb (CAB18423) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 300s.
