The SF3B4 Polyclonal Antibody (CAB17608) is an essential tool for researchers studying SF3B4, a key protein involved in RNA splicing. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the SF3B4 protein, researchers can detect and analyze its expression in various cell types, making it an ideal choice for studies in molecular biology and RNA processing.SF3B4 is a crucial component of the spliceosome complex, responsible for accurately removing introns from pre-mRNA molecules during gene expression.
Dysregulation of SF3B4 has been implicated in various human diseases, including cancers and genetic disorders. By studying the function and regulation of SF3B4, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify novel therapeutic targets. The SF3B4 Polyclonal Antibody is a valuable tool for advancing research in this field and uncovering new possibilities for treating diseases associated with RNA processing abnormalities.
Product Name:
SF3B4 Rabbit Polyclonal Antibody
SKU:
CAB17608
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SF3B4 (NP_005841.1).
This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.
Purification Method:
Affinity purification
Gene ID:
10262
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SF3B4 Rabbit pAb (CAB17608) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
