The SEC31A Polyclonal Antibody (CAB9321) is a valuable tool for research involving SEC31A, a key protein involved in vesicle trafficking and protein transport within the cell. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the SEC31A protein, this antibody enables researchers to detect and analyze SEC31A in various cell types, making it an essential reagent for studies in cell biology and molecular biology.SEC31A is a critical component of the COPII complex, which is responsible for the formation of transport vesicles that carry proteins from the endoplasmic reticulum to the Golgi apparatus.
Dysfunction in SEC31A can lead to defects in protein transport and secretion, potentially contributing to various diseases such as neurodegenerative disorders and metabolic syndromes. Understanding the role of SEC31A in cellular processes is crucial for advancing our knowledge of these diseases and developing targeted therapies.
Product Name:
SEC31A Rabbit Polyclonal Antibody
SKU:
CAB9321
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-500 of human SEC31A (NP_055748.2).
The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
22872
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SEC31A Rabbit pAb (CAB9321) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
