The RYR3 Polyclonal Antibody (PAC060809) is a valuable tool for researchers studying the ryanodine receptor 3 (RYR3) protein, which plays a crucial role in calcium ion homeostasis and muscle contraction. This antibody, produced in rabbits, has been validated for use in various applications, including Western blotting and immunohistochemistry.RYR3 is primarily expressed in skeletal muscle and certain regions of the brain, where it regulates calcium release from intracellular stores.
Dysregulation of RYR3 has been implicated in various muscle disorders, neurodegenerative diseases, and cardiac arrhythmias. By targeting RYR3 with this antibody, researchers can investigate its role in these conditions and develop potential therapeutic interventions.Overall, the RYR3 Polyclonal Antibody is a reliable tool for studying the function and regulation of RYR3, providing valuable insights into its involvement in physiological processes and disease pathology.
IHC image of PACO60809 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO60809 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm in muscle and thereby plays a role in triggering muscle contraction. May regulate Ca(2+) release by other calcium channels. Calcium channel that mediates Ca(2+)-induced Ca(2+) release from the endoplasmic reticulum in non-muscle cells. Contributes to cellular calcium ion homeostasis (By similarity). Plays a role in cellular calcium signaling.
Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm in muscle and thereby plays a role in triggering muscle contraction. May regulate Ca2+ release by other calcium channels. Calcium channel that mediates Ca2+-induced Ca2+ release from the endoplasmic reticulum in non-muscle cells. Contributes to cellular calcium ion homeostasis (). Plays a role in cellular calcium signaling.
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a ryanodine receptor, which functions to release calcium from intracellular storage for use in many cellular processes. For example, the encoded protein is involved in skeletal muscle contraction by releasing calcium from the sarcoplasmic reticulum followed by depolarization of T-tubules. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
