The RUNX1 Polyclonal Antibody offered by Assay Genie (catalog number ARG53656) is a valuable tool for researchers studying the RUNX1 protein, a transcription factor involved in hematopoiesis and the development of blood cells. This antibody, also raised in rabbits, is highly specific to human samples and has been validated for use in various applications, including immunohistochemistry and flow cytometry.The RUNX1 protein, also known as AML1, is a key player in the regulation of hematopoietic stem cells and progenitor cells, making it a pivotal target for studies in hematological disorders such as leukemia. Its role in normal blood cell development and its involvement in leukemia make it a prime candidate for research aimed at understanding the mechanisms underlying these diseases and potentially identifying new therapeutic targets.
By using the RUNX1 Polyclonal Antibody, researchers can detect and analyze the expression of the RUNX1 protein in different cell types and tissues, providing valuable insights into its function and regulatory mechanisms. This antibody is a valuable tool for advancing research in the fields of hematology, oncology, and stem cell biology, ultimately contributing to the development of new diagnostic and therapeutic strategies for hematological disorders.
Product Name:
RUNX1 Polyclonal Antibody
SKU:
CAB24747
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RUNX1.
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
861
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using RUNX1 Rabbit pAb (CAB24747) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC): SH-SY5Y.Exposure time: 60s.
