The RTCA Polyclonal Antibody (PAC035094) is intended for use in research involving RTCA, a protein involved in cell regulation and signaling pathways. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding specifically to the RTCA protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a valuable tool for studies in cell biology and signal transduction research.
RTCA, also known as Regulator of Telomere Elongation Helicase 1, plays a crucial role in the regulation of telomere length and cellular senescence. Dysregulation of RTCA has been implicated in aging-related diseases and cancer development, making it a promising target for therapeutic interventions. Studying the function of RTCA is essential for uncovering its role in disease progression and identifying potential treatment strategies aimed at targeting this pathway.
Antibody Name:
RTCA Antibody (PACO35094)
Antibody SKU:
PACO35094
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human RNA 3'-terminal phosphate cyclase protein (167-379AA)
Immunohistochemistry analysis of human breast cancer using PACO35094 at dilution of 1:100.
Background:
Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
RTCD1: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'- phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Ligase; EC 6.5.1.4; RNA-bindingChromosomal Location of Human Ortholog: 1p21.2Cellular Component: nucleoplasm; nucleusMolecular Function: RNA binding; RNA-3'-phosphate cyclase activity; ATP bindingBiological Process: RNA processing
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
