Anti-RRM2 Antibody (CAB5255)
- SKU:
- CAB5255
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Anti-RRM2 Antibody (CAB5255)
The RRM2 Polyclonal Antibody (CAB5255) is a valuable tool for researchers studying the RRM2 protein, which plays a crucial role in DNA synthesis and repair. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the RRM2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for studies in molecular biology and cancer research.
RRM2 is a key enzyme involved in the production of nucleotides necessary for DNA replication and repair. Dysregulation of RRM2 expression has been linked to various diseases, including cancer, making it a promising target for therapeutic development. By uncovering the mechanisms and functions of RRM2, researchers can gain valuable insights into disease progression and potentially identify new treatment strategies.
| Antibody Name: | Anti-RRM2 Antibody |
| Antibody SKU: | CAB5255 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human RRM2 (NP_001025.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:10 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF-7, SW480, HeLa, Jurkat, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human RRM2 (NP_001025.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLSL RVPL APIT DPQQ LQLS PLKG LSLV DKEN TPPA LSGT RVLA SKTA RRIF QEPT EPKT KAAA PGVE DEPL LREN PRRF VIFP IEYH DIWQ MYKK AEAS FWTA EEVD LSKD IQHW ESLK PEER YFIS HVLA FFAA SDGI VNEN LVER FSQE VQIT EARC FYGF QIAM ENIH SEMY SLLI DTYI KDPK EREF LFNA IETM PCVK KKAD WALR WIGD KEAT YGER VVAF AAVE GIFF SGSF ASIF WLKK RGLM PGLT FSNE LISR DEGL HCDF ACLM FKHL VHKP SEER VREI IINA VRIE QEFL TEAL PVKL IGMN CTLM KQYI EFVA DRLM LELG FSKV FRVE NPFD FMEN ISLE GKTN FFEK RVGE YQRM GVMS SPTE NSFT LDAD F |
| Gene ID: | 6241 |
| Uniprot: | P31350 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 44kDa/51kDa |
| Observed MW: | 45kDa |
| Synonyms: | RRM2, R2, RR2, RR2M |
| Background: | This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. |
| UniProt Protein Function: | RRM2: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. Belongs to the ribonucleoside diphosphate reductase small chain family. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - glutathione; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 1.17.4.1; Oxidoreductase Chromosomal Location of Human Ortholog: 2p25-p24 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:protein binding; metal ion binding; ribonucleoside-diphosphate reductase activity Biological Process: G1/S-specific transcription in mitotic cell cycle; deoxyribonucleoside diphosphate metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; deoxyribonucleotide biosynthetic process; protein heterotetramerization; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle |
| NCBI Summary: | This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009] |
| UniProt Code: | P31350 |
| NCBI GenInfo Identifier: | 400979 |
| NCBI Gene ID: | 6241 |
| NCBI Accession: | P31350.1 |
| UniProt Secondary Accession: | P31350,Q5WRU7, B2R9B5, J3KP43, |
| UniProt Related Accession: | P31350 |
| Molecular Weight: | 389 |
| NCBI Full Name: | Ribonucleoside-diphosphate reductase subunit M2 |
| NCBI Synonym Full Names: | ribonucleotide reductase M2 |
| NCBI Official Symbol: | RRM2Â Â |
| NCBI Official Synonym Symbols: | R2; RR2; RR2MÂ Â |
| NCBI Protein Information: | ribonucleoside-diphosphate reductase subunit M2; ribonucleotide reductase small chain; ribonucleotide reductase small subunit; ribonucleotide reductase M2 polypeptide |
| UniProt Protein Name: | Ribonucleoside-diphosphate reductase subunit M2 |
| UniProt Synonym Protein Names: | Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit |
| Protein Family: | Ribonucleoside-diphosphate reductase |
| UniProt Gene Name: | RRM2Â Â |
| UniProt Entry Name: | RIR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RRM2 antibody (CAB5255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of A549 cells using RRM2 antibody (CAB5255). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug RRM2 antibody (CAB5255). Western blot was performed from the immunoprecipitate using RRM2 antibody (CAB5255) at a dilition of 1:1000. |
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