The RPA1 Polyclonal Antibody (CAB0874) is a valuable tool for researchers studying RPA1, a vital protein involved in DNA replication and repair processes. This antibody, specifically developed for use in Western blot applications, offers high reactivity with human samples, allowing for accurate detection and analysis of RPA1 in various cell types.RPA1, also known as Replication Protein A1, plays a crucial role in maintaining DNA integrity and stability, making it a key player in cellular processes such as DNA replication, DNA damage response, and cell cycle regulation.
Dysregulation of RPA1 has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions and diagnostic purposes.By utilizing the RPA1 Polyclonal Antibody, researchers can gain insights into the function and regulation of RPA1, furthering our understanding of DNA repair mechanisms and their implications in disease development. This antibody is an essential tool for studies in molecular biology, oncology, and genetics, providing valuable information for advancing research in these fields.
Product Name:
RPA70/RPA1 Rabbit Polyclonal Antibody
SKU:
CAB0874
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPA70/RPA70/RPA1 (NP_002936.1).
This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
Purification Method:
Affinity purification
Gene ID:
6117
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPA70/RPA1 Rabbit pAb (CAB0874) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
