The ROBO2 Polyclonal Antibody (CAB10070) is a valuable tool for researchers studying the ROBO2 gene, which encodes a membrane protein involved in axon guidance and cell migration during development. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in a variety of applications, including Western blot and immunohistochemistry.ROBO2 is a key player in the nervous system, particularly in guiding the growth of axons and dendrites to their correct destinations during development.
Dysregulation of ROBO2 expression has been implicated in neurological disorders and cancers, making it an important target for further investigation. By using the ROBO2 Polyclonal Antibody, researchers can accurately detect and study ROBO2 protein levels in various cell types and tissues, advancing our understanding of its role in nervous system development and disease pathology.
Product Name:
ROBO2 Rabbit Polyclonal Antibody
SKU:
CAB10070
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 450-660 of human ROBO2 (NP_001122401.1).
The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6092
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ROBO2 antibody (CAB10070) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
