The RNF43 Polyclonal Antibody (PAC057948) is a valuable tool for researchers studying the RNF43 protein, a key player in the Wnt signaling pathway. This antibody is produced in rabbits and exhibits high reactivity with human samples, enabling reliable detection of RNF43 in Western blot applications. By specifically binding to the RNF43 protein, this antibody facilitates detailed analysis of its function and expression in various cell types.RNF43 is a transmembrane E3 ubiquitin ligase that serves as a negative regulator of the Wnt signaling pathway, which is involved in cell proliferation, differentiation, and stem cell maintenance.
Dysregulation of Wnt signaling, often associated with mutations in Wnt pathway components like RNF43, has implications in various diseases including cancer and developmental disorders. Investigating the role of RNF43 in these contexts can provide valuable insights into disease mechanisms and potential therapeutic targets.The RNF43 Polyclonal Antibody is a versatile tool for researchers in the fields of cancer biology, developmental biology, and stem cell research, offering reliable and specific detection of RNF43 to enhance understanding of its function and potential implications in disease.
Antibody Name:
RNF43 Antibody (PACO57948)
Antibody SKU:
PACO57948
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:500-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human E3 ubiquitin-protein ligase RNF43 protein (566-697AA)
IHC image of PACO57948 diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.
RNF43: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Belongs to the ZNRF3 family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Membrane protein, integralChromosomal Location of Human Ortholog: 17q22Cellular Component: integral to plasma membrane; plasma membraneMolecular Function: frizzled binding; protein binding; ubiquitin-protein ligase activityBiological Process: negative regulation of Wnt receptor signaling pathway; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
