The RGA1 Polyclonal Antibody (CAB19214) is a valuable tool for researchers studying the RGA1 protein, a key regulator of plant growth and development. This antibody, produced in rabbits, has been shown to be highly specific and sensitive in detecting RGA1 in plant samples, making it ideal for use in various experimental techniques such as Western blot and immunohistochemistry.RGA1 is a critical gene involved in plant signaling pathways that control responses to environmental stimuli, hormone signaling, and stress tolerance. Understanding the function and regulation of RGA1 is essential for improving crop yield, developing stress-resistant crops, and enhancing agricultural sustainability.
The RGA1 Polyclonal Antibody facilitates the study of RGA1 expression patterns and localization in different plant tissues, providing valuable insights into its role in plant biology.By using the RGA1 Polyclonal Antibody in their research, scientists can explore the molecular mechanisms underlying RGA1-mediated processes and advance our understanding of plant growth and adaptation in changing environmental conditions. This antibody is a reliable tool for investigations in plant biology, genetics, and biotechnology, offering researchers a precise and effective means of studying the functions of the RGA1 protein.
Product Name:
RGA1 Polyclonal Antibody
SKU:
CAB19214
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Oryza sativa
Immunogen:
Recombinant protein of Oryza sativa RGA1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
D1; GA1; GPA1; RGA1; GP-alpha-1; OJ1005_D04.15
Positive Sample:
Rice root,Rice leaf
Cellular Localization:
plasma membrane
Calculated MW:
44kDa
Observed MW:
44.2kDa
This? gene Ubiquitous expression in leaves before flowering (RPKM 48.7), leaves after flowering (RPKM 36.4) and 6 other tissues. It encodes protein Alpha subunit of G proteins (guanine nucleotide binding). G-alpha has three isomers, guanine nucleotide-binding protein alpha-1 subunit isoform X1，guanine nucleotide-binding protein alpha-1 subunit isoform X2，and guanine nucleotide-binding protein alpha-1 subunit isoform X3.RGA1 is one of the regulators in salt response partially through ROS scavenging, which might be helpful in elucidating salt tolerant mechanisms of heterotrimeric G protein in rice(PMID 30621186). The α-subunit of the rice heterotrimeric G protein, RGA1, regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1 (PMID 27194741).? The Gα heterotrimeric G protein subunit, RGA1,? plays an important role in regulating photoprotection and photoprotection of rice (PMID 29216416).
Purification Method:
Affinity purification
Gene ID:
4338448
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RGA1 Rabbit pAb (CAB19214) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:8000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
