The RFX6 Polyclonal Antibody (PACO37726) is a valuable tool for researchers studying the transcription factor RFX6, which plays a crucial role in the development and function of pancreatic islet cells. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in various applications, including Western blot and immunofluorescence.RFX6 is known for its involvement in regulating the expression of genes essential for pancreatic development, insulin production, and glucose metabolism. Dysregulation of RFX6 has been linked to disorders such as diabetes mellitus, making it a promising target for further investigation in both basic research and clinical applications.
By using the RFX6 Polyclonal Antibody, researchers can study the localization and expression levels of RFX6 in different cell types and tissues, leading to a better understanding of its function and potential therapeutic implications. This antibody is a valuable tool for advancing research in the fields of diabetes, endocrinology, and developmental biology.
Antibody Name:
RFX6 Antibody (PACO37726)
Antibody SKU:
PACO37726
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human DNA-binding protein RFX6 protein (1-260AA)
Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO37726 at dilution of 1:100.
Background:
Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes. Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription.
Synonyms:
DNA-binding protein RFX6 (Regulatory factor X 6) (Regulatory factor X domain-containing protein 1), RFX6, RFXDC1
UniProt Protein Function:
Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin (PubMed:20148032, PubMed:25497100). Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed:20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (PubMed:25497100).
UniProt Protein Details:
NCBI Summary:
The nuclear protein encoded by this gene is a member of the regulatory factor X (RFX) family of transcription factors. Studies in mice suggest that this gene is specifically required for the differentiation of islet cells for the production of insulin, but not for the differentiation of pancreatic polypeptide-producing cells. It regulates the transcription factors involved in beta-cell maturation and function, thus, restricting the expression of the beta-cell differentiation and specification genes. Mutations in this gene are associated with Mitchell-Riley syndrome, which is characterized by neonatal diabetes with pancreatic hypoplasia, duodenal and jejunal atresia, and gall bladder agenesis.[provided by RefSeq, Sep 2010]
