UL16-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor; NKG2D. Ten members of the human ULBP/RAET1 gene family have been identified to encode for potentially functional proteins; and have tissue-specific expressions. ULBP1; also known as RAET1I and NKG2DL1; together with at least ULBP 2 and 3; are well-known ligands for NKG2D; and activate multiple signaling pathways in primary NK cells; resulting in the production of cytokines and chemokines. ULBP1 is expressed in T-cells; B-cells; erythroleukemia cell lines and in a wide range of tissues including heart; brain; lung; liver and bone marrow; as well as some tumor cells. As an unconventional member of the MHC class I family; ULBP1 function in immune responses; especially in cancer and infectious diseases. Unlike other ULBP members; ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor; and thus might escape the immune surveillance. Furthermore; UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.
Product Name:
Human ULBP1/N2DL1 Recombinant Protein (RPES3461)
Product Code:
RPES3461
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
RAET1I
Accession:
NP_079494.1
Sequence:
Met 1-Gly 216
Fusion tag:
C-His
Activity:
Immobilized human ULBP1-His at 10 µg/ml (100 ?l/well) can bind human NKG2D;The EC50 of human NKG2D is 0.39-0.91 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human ULBP1 (NP_079494.1) (Met 1-Gly 216) was expressed; with a polyhistidine tag at the C-terminus.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
23.8 kDa
AP Mol Mass:
28-32 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
