Ubiquitin carboxyl-terminal hydrolase 7 (Ubiquitin thioesterase 7/Herpesvirus-associated ubiquitin-specific protease/Ubiquitin-specific-processing protease 7/USP7/HAUSP) is a widely expressed protein which belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the control of stress response pathways, epigenetic silencing, and viral infection progression. Cysteine peptidase core, N- and C-terminus domains required for protein-protein interactions are present in USP7. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. USP7 deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. ICP0, the Herpes Simplex Virus Type 1 (HSV-1) regulatory protein, stimulates lytic replication and reactivation of latent viral genes. ICP0 interacts very strongly with USP7. During productive HSV-1 infection, ICP0 stability is enhanced by USP7-dependent stabilization of ICP0, which overpowers ICP0 breakdown caused by USP7. The biological significance of the ICP0-USP7 relationship may be most apparent in natural infection situations, when little amounts of ICP0 are produced. Human SETD8/PR-Set7 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human SETD8/PR-Set7 Recombinant Protein (RPES0931)
Product Code:
RPES0931
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
KMT5A,PR-Set7,SET07,SET8
Accession:
NP_065115.3
Sequence:
Lys 195-His 352
Fusion tag:
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human SETD8 (NP_065115.3) (Lys 195-His 352) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
18.2 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 100mM NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
