Recombinant Human Lipocalin-2/NGAL/LCN2 Protein (RPCB0006)
- SKU:
- RPCB0006
- Size:
- 10µg
- Tag:
- C-His
- Reactivity:
- Human
Description
| Product Name: | Recombinant Human Lipocalin-2/NGAL/LCN2 Protein |
| SKU: | RPCB0006 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Lipocalin-2/NGAL/LCN2 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.01 EU/μg of the protein by LAL method. |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 3934 |
Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules . It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) . Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation . Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis . Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant . The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) . Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron . Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway .
| Storage: | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P80188 |
 | Recombinant Human Lipocalin-2/NGAL/LCN2 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions. , showing single bands at 20-25 kDa and 20-25,40-45 kDa. respectively. |
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