Recombinant Human IFN-alpha 4 Protein (RPCB1686)
- SKU:
- RPCB1686
- Size:
- 20µg
- Tag:
- C-His
- Reactivity:
- Human
Description
| Product Name: | Recombinant Human IFN-alpha 4 Protein |
| SKU: | RPCB1686 |
| Size: | 20µg |
| Tag: | C-His |
| Reactivity: | Human |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human IFN-alpha 4 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1EU/μg of the protein by LAL method |
| Purity: | > 90% by SDS-PAGE. |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 3441 |
IFN-alpha 4,Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors . The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling . Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them . The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle. mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly .
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P05014 |
 | Recombinant Human IFN-alpha 4 Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 15-20 kDa. |
 | Recombinant Human IFN-alpha 4 Protein cytotoxicity assay using TF-1 cells. The ED₅₀ for this effect is 0.26_x0000_1.02 ng/mL, corresponding to a specific activity of 9.80×105~3.85×106 units/mg. |
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