Vascular endothelial growth factor (VEGF) is a protein that promotes angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family, and it usually forms a dimer via disulfide bonds. VEGF-A protein is a glycosylated mitogen that acts specifically on endothelial cells and has diverse functions, including enhancing vascular permeability, promoting angiogenesis, vasculogenesis and endothelial cell development, promoting migration of cells, inhibiting apoptosis and tumor progression. VEGF-A protein is also a vasodilator that increases microvascular permeability; thus it was originally referred to as vascular permeability factor. VEGFA/VEGF165 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).The ED50 for this effect is typically 4-16 ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human VEGF165 isoform (P15692-4) (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
19.2 kDa
AP Mol Mass:
20 and 22 kDa
Formulation:
Lyophilized from sterile 100 mM Glycine; 10 mM NaCl; pH 7.0.1. Normally 5 % - 8 % trehalose; mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
