The RB1 Polyclonal Antibody (CAB16966) is a vital tool for researchers studying the retinoblastoma protein (RB1) and its role in cell cycle regulation and tumorigenesis. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting RB1 in human samples, making it a reliable choice for Western blot applications.RB1 is a tumor suppressor protein that plays a crucial role in cell division and growth control. Mutations in the RB1 gene have been linked to various cancers, including retinoblastoma, osteosarcoma, and breast cancer. By targeting RB1 with this polyclonal antibody, researchers can accurately monitor RB1 expression levels in different cell types, providing valuable insights into cell cycle regulation and cancer progression.
With its versatility and effectiveness in detecting RB1, the CAB16966 antibody is an essential tool for advancing our understanding of tumorigenesis mechanisms and developing targeted therapies for RB1-related cancers. Researchers in oncology, cell biology, and molecular biology can benefit from incorporating this antibody into their studies to unravel the complex role of RB1 in cancer development.
Product Name:
RB Rabbit Polyclonal Antibody
SKU:
CAB16966
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 701-928 of human RB (NP_000312.2).
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method:
Affinity purification
Gene ID:
5925
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RB Rabbit pAb (CAB16966) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
