Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit
The Rat TCC/C5b-9 Terminal Complement Complex (C5b-9) ELISA Kit is designed for the precise measurement of C5b-9 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.The C5b-9 terminal complement complex is a crucial component of the complement system, playing a key role in immune response and inflammation. Dysregulation of the complement system has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases, making the measurement of C5b-9 levels vital for understanding disease pathogenesis and developing potential therapeutics.
With its advanced technology and high-quality components, the Rat TCC/C5b-9 Terminal Complement Complex ELISA Kit provides researchers with a valuable tool for studying the complement system and its involvement in health and disease.
Product Name:
Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit
SKU:
RTES00792
Target:
Rat TCC C5b-9 (Terminal Complement Complex C5b-9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sC5b-9. You can calculate the concentration of Rat sC5b-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
89-103
87-100
Average (%)
97
96
93
1:4
Range (%)
90-106
81-93
88-100
Average (%)
98
86
93
1:8
Range (%)
85-99
79-94
83-96
Average (%)
92
86
90
1:16
Range (%)
93-104
86-101
86-99
Average (%)
99
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.25
3.79
0.51
1.14
4.0
Standard deviation
0.04
0.07
0.16
0.03
0.07
0.18
C V (%)
7.41
5.6
4.22
5.88
6.14
4.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat sC5b-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat sC5b-9 in samples. No significant cross-reactivity or interference between Rat sC5b-9 and analogues was observed.
