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Rat Serum Amyloid P Component (SAP) ELISA Kit (RTDL00926)

SKU:
RTDL00926
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P23680
Sensitivity:
2.15ng/mL
Range:
6.25-400ng/mL
ELISA Type:
Sandwich
Synonyms:
APCS,PTX2,Pentaxin-Related,9.5S Alpha-1-Glycoprotein
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649

Description

system_update_altDatasheet

Rat Serum Amyloid P Component (SAP) ELISA Kit

The Rat Serum Amyloid P Component (SAP) ELISA Kit is specifically designed for the precise detection of SAP levels in rat serum samples. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for research purposes. SAP is an important protein that plays a crucial role in the immune response and inflammation processes. It is involved in the clearance of amyloid fibrils and is associated with various diseases, including Alzheimer's disease and systemic amyloidosis.

Thus, monitoring SAP levels can provide valuable insights into the development and progression of these conditions and aid in the development of potential therapeutic interventions.Overall, the Rat Serum Amyloid P Component (SAP) ELISA Kit is a valuable tool for researchers studying immunology, inflammation, and related diseases in rat models.

Product Name:Rat Serum Amyloid P Component (SAP) ELISA Kit
Product Code:RTDL00926
Size:96 Assays
Target:SAP
Synonyms:APCS, PTX2, Pentaxin-Related, 9.5S Alpha-1-Glycoprotein
Detection Method:Sandwich
Range:6.25-400ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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