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Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit

SKU:
RTDL01086
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29598
Sensitivity:
5.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
PLAU,ATF,URK,UK,UP-A,Abbokinase,Urokinase-Type Plasminogen Activator
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit

The Rat Plasminogen Activator Urokinase (UPA) ELISA Kit is a reliable and accurate method for detecting levels of UPA in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Plasminogen activator urokinase (UPA) is an important enzyme involved in fibrinolysis and extracellular matrix remodeling, playing a crucial role in processes such as wound healing, tissue repair, and cancer metastasis.

Monitoring UPA levels can provide valuable insights into the progression of various diseases and help in the development of targeted therapies.Overall, the Rat Plasminogen Activator Urokinase (UPA) ELISA Kit is a valuable tool for researchers studying the role of UPA in various physiological and pathological conditions, providing accurate and reliable data for further investigations.

Product Name:Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit
Product Code:RTDL01086
Size:96 Assays
Target:uPA
Synonyms:PLAU, ATF, URK, UK, UP-A, Abbokinase, Urokinase-Type Plasminogen Activator
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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