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Rat Interleukin 23 Receptor (IL-23R) ELISA Kit

SKU:
RTDL00559
Product Type:
ELISA Kit
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Interleukin 23 Receptor (IL-23R) ELISA Kit

The Rat Interleukin-23 Receptor (IL23R) ELISA Kit is specifically designed for the accurate measurement of IL23R levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and precision, ensuring dependable and consistent results for various research applications.IL23R is a critical receptor involved in immune responses, particularly in the regulation of pro-inflammatory cytokines.

Dysregulation of IL23R has been associated with autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a potential therapeutic target.With its reliable performance and broad applicability, the Rat IL23R ELISA Kit is an indispensable tool for researchers studying the role of IL23R in health and disease, providing valuable insights into potential treatment strategies and diagnostic markers.

Product Name:Rat Interleukin 23 Receptor (IL-23R) ELISA Kit
Product Code:RTDL00559
Size:96 Assays
Target:IL23R
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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