Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit
The Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit is a specialized assay designed for the precise and quantitative detection of ICAM-1 levels in various rat biological samples. ICAM-1 is a crucial cell surface glycoprotein involved in mediating cell adhesion and immune responses. Its expression and regulation are of significant interest in the context of inflammation, immune cell interactions, and various pathological conditions.
Accurate quantification of ICAM-1 levels using this ELISA kit is essential for elucidating its role in cell adhesion processes, inflammatory responses, and disease pathogenesis. Researchers can harness the data obtained from this assay to gain insights into the mechanisms underlying immune cell communication, inflammatory disorders, and the development of potential therapeutic interventions targeting ICAM-1 pathways. With its exceptional sensitivity and specificity, the Rat ICAM-1/CD54 ELISA Kit from Assay Genie ensures reliable and reproducible results. Manufactured according to stringent quality control standards, this kit offers robust performance and ease of use, making it a valuable tool for research studies focused on understanding the function and implications of ICAM-1 in a variety of biological contexts.
Product Name:
Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit
SKU:
AEES00515
Target:
Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ICAM-1/CD54. You can calculate the concentration of Rat ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-98
100-110
88-102
Average (%)
95
103
96
1:4
Range (%)
90-104
88-98
93-105
Average (%)
97
91
98
1:8
Range (%)
91-102
96-107
89-100
Average (%)
95
100
96
1:16
Range (%)
89-98
88-103
93-102
Average (%)
92
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-109
105
EDTA plasma (n=5)
94-105
98
Cell culture media (n=5)
94-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.9
2.33
9.21
0.89
2.08
9.86
Standard deviation
0.05
0.11
0.46
0.05
0.1
0.5
C V (%)
5.56
4.72
4.99
5.62
4.81
5.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Rat ICAM-1/CD54 and analogues was observed.
