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Rat Fibroblast Growth Factor 3 (FGF3) ELISA Kit

SKU:
RTDL00381
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8R5L9
Sensitivity:
5.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
HBGF3,INT2,Heparin-binding growth factor 3,Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog,INT-2 Proto-Oncogene Protein,Proto-oncogene Int-2
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Fibroblast Growth Factor 3 (FGF3) ELISA Kit

The Rat Fibroblast Growth Factor 3 (FGF3) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of FGF3 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for various research applications.FGF3 is a key growth factor that plays a critical role in cell growth, proliferation, and differentiation. It is involved in various biological processes, including embryonic development, tissue repair, and wound healing.

Dysregulation of FGF3 signaling has been implicated in several diseases, such as cancer and developmental disorders, highlighting its importance as a potential therapeutic target.By using the Rat FGF3 ELISA Kit, researchers can gain valuable insights into the role of FGF3 in normal physiological processes and disease pathology. This kit offers a simple and efficient way to quantify FGF3 levels, allowing for in-depth studies and the development of novel therapeutic strategies.

Product Name:Rat Fibroblast Growth Factor 3 (FGF3) ELISA Kit
Product Code:RTDL00381
Size:96 Assays
Target:FGF3
Synonyms:HBGF3, INT2, Heparin-binding growth factor 3, Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog, INT-2 Proto-Oncogene Protein, Proto-oncogene Int-2
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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