The Rat F8 (Coagulation Factor 8) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of Coagulation Factor 8 levels in rat samples such as serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it an ideal tool for research studies related to coagulation and hemostasis.Coagulation Factor 8 is a key factor in the blood clotting cascade, playing a crucial role in preventing excessive bleeding and maintaining hemostasis.
Dysregulation of Coagulation Factor 8 is associated with various bleeding disorders and coagulopathies, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat F8 (Coagulation Factor 8) CLIA Kit offers researchers a powerful tool for investigating the role of Coagulation Factor 8 in physiological and pathological processes, providing valuable insights into coagulation disorders and potential treatment strategies.
Product Name:
Rat F8 (Coagulation Factor 8) CLIA Kit
SKU:
RTES00131
Target:
Rat F8 (Coagulation Factor 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat F13. You can calculate the concentration of Rat F13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
101-116
94-110
Average (%)
94
106
102
1:4
Range (%)
84-100
97-111
89-102
Average (%)
91
103
94
1:8
Range (%)
90-105
94-111
99-113
Average (%)
96
101
107
1:16
Range (%)
95-110
94-111
97-111
Average (%)
102
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
377.64
1270.9
3215.69
366.85
1382.9
3410.54
Standard deviation
39.88
144.25
271.4
42.04
116.16
337.3
C V (%)
10.56
11.35
8.44
11.46
8.4
9.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat F13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat F13 in samples. No significant cross-reactivity or interference between Rat F13 and analogues was observed.
