The Rat C-P (C-peptide) ELISA Kit is specifically designed to empower researchers with the precise and quantitative analysis of C-peptide levels in a variety of biological samples collected from rat models. C-peptide, a crucial peptide derived from proinsulin during insulin biosynthesis, serves as a valuable indicator of endogenous insulin production and pancreatic beta-cell function. By accurately measuring C-peptide concentrations, this ELISA kit facilitates a comprehensive examination of pancreatic beta-cell activity, insulin secretion, and glucose metabolism in rat subjects.
Accurate quantification of C-peptide levels is essential for providing insights into pancreatic beta-cell health, insulin secretion patterns, and overall glucose homeostasis. The C-P ELISA kit enables researchers to explore the intricate interplay between C-peptide, insulin, and glucose regulation, offering a deeper understanding of metabolic processes and potential pathological conditions related to diabetes and other metabolic disorders. Manufactured with a focus on precision and reliability, the Rat C-P ELISA Kit ensures exceptional sensitivity, specificity, and reproducibility, guaranteeing robust performance and consistent results. Researchers can rely on this kit to deliver accurate and insightful data, making it an indispensable tool for investigations into pancreatic function and metabolic health in rat models.
Product Name:
Rat C-P (C-peptide) ELISA Kit
SKU:
AEES00520
Target:
Rat C-P (C-peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C-P. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C-P. You can calculate the concentration of Rat C-P in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
97-105
100-113
Average (%)
98
102
105
1:4
Range (%)
96-110
83-99
93-106
Average (%)
103
91
100
1:8
Range (%)
91-101
93-100
89-98
Average (%)
96
96
94
1:16
Range (%)
89-98
95-103
90-106
Average (%)
95
100
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
92-101
95
Cell culture media (n=5)
96-104
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.4
101.02
462.16
43.32
100.95
494.3
Standard deviation
2.88
4.79
23.62
2.3
4.59
26.0
C V (%)
5.83
4.74
5.11
5.31
4.55
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat C-P concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat C-P in samples. No significant cross-reactivity or interference between Rat C-P and analogues was observed.
