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Rat Atrial Natriuretic Peptide (ANP) ELISA Kit

SKU:
RTDL00081
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B0BMW5
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ANH,ANF,CDD,Atrial Natriuretic Factor,Atrial Natriuretic Hormone,Atriopeptin,Cardionatrine,Cardiodilatin
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Atrial Natriuretic Peptide (ANP) ELISA Kit

The Rat Atrial Natriuretic Peptide (ANP) ELISA Kit is a powerful tool for accurately measuring ANP levels in rat samples including serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.ANP is a hormone secreted by the heart in response to stretching of the atria, playing a crucial role in regulating blood pressure and fluid balance.

Elevated ANP levels have been associated with conditions such as heart failure, hypertension, and kidney disease, making it a valuable biomarker for studying these diseases and evaluating potential treatment options.Overall, the Rat ANP ELISA Kit is an essential tool for researchers investigating the role of ANP in cardiovascular health and disease, providing valuable insights into physiological and pathological processes related to cardiac function.

Product Name:Rat Atrial Natriuretic Peptide (ANP) ELISA Kit
Product Code:RTDL00081
Size:96 Assays
Target:ANP
Synonyms:ANH, ANF, CDD, Atrial Natriuretic Factor, Atrial Natriuretic Hormone, Atriopeptin, Cardionatrine, Cardiodilatin
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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