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Rat Apolipoprotein A1 (APOA1) ELISA Kit

SKU:
RTDL00091
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04639
Sensitivity:
8.5ng/mL
Range:
23.437-1500ng/mL
ELISA Type:
Sandwich
Synonyms:
Apo-A1,ApoA-1 Milano,ProapoA-I,Proapolipoprotein A-I,Truncated apolipoprotein A-I
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Apolipoprotein A1 (APOA1) ELISA Kit

The Rat Apolipoprotein A1 (APOA1) ELISA Kit is specifically designed for the precise measurement of APOA1 levels in rat serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results, making it a valuable tool for various research purposes.APOA1 is a critical protein involved in lipid metabolism and is a major component of high-density lipoprotein (HDL) particles.

It plays a crucial role in maintaining cholesterol homeostasis and reducing the risk of cardiovascular diseases. Studying APOA1 levels can provide important insights into lipid disorders and cardiovascular health, making this kit essential for researchers investigating these conditions and potential therapeutic interventions.

Product Name:Rat Apolipoprotein A1 (APOA1) ELISA Kit
Product Code:RTDL00091
Size:96 Assays
Target:APOA1
Synonyms:Apo-A1, ApoA-1 Milano, ProapoA-I, Proapolipoprotein A-I, Truncated apolipoprotein A-I
Detection Method:Sandwich
Range:23.4-1500ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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