The RAG2 Polyclonal Antibody (CAB5626) is a valuable research tool for studying RAG2, a key protein involved in V(D)J recombination and immune system development. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By specifically binding to RAG2, this antibody enables researchers to detect and analyze the protein in various cell types, making it ideal for studies in immunology and hematological research.RAG2 is essential for the rearrangement of immune system genes, playing a crucial role in the generation of diverse antigen receptors required for immune responses.
Understanding the function and regulation of RAG2 is important for unraveling the mechanisms underlying immune system development and function. This antibody provides a valuable tool for investigating the role of RAG2 in diseases related to immunodeficiency, leukemia, and autoimmune disorders, paving the way for the development of new therapeutic strategies targeting this protein.
Product Name:
[KO Validated] RAG2 Rabbit Polyclonal Antibody
SKU:
CAB5626
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 351-527 of human RAG2 (NP_000527.2).
This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
Purification Method:
Affinity purification
Gene ID:
5897
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] RAG2 Rabbit pAb (CAB5626) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
