The RABGEF1 Polyclonal Antibody (PAC041818) is a valuable tool for researchers studying RABGEF1, a key protein involved in the regulation of intracellular trafficking and vesicle transport. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, and has been validated for Western blot applications.RABGEF1, also known as RAB Guanine Nucleotide Exchange Factor 1, plays a crucial role in facilitating the activation of small GTPases, which are essential for regulating various cellular processes such as membrane trafficking, cell signaling, and cytoskeletal dynamics.
Dysregulation of RABGEF1 has been implicated in diseases such as cancer, neurodegenerative disorders, and infectious diseases.By using the RABGEF1 Polyclonal Antibody, researchers can detect and analyze the expression levels of RABGEF1 in different cell types and experimental conditions, providing valuable insights into its functions and potential therapeutic targets. This antibody is a valuable tool for studies in cell biology, molecular biology, and disease mechanisms, contributing to advancements in biomedical research and drug development.
Antibody Name:
RABGEF1 Antibody (PACO41818)
Antibody SKU:
PACO41818
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Rab5 GDP/GTP exchange factor protein (1-491AA)
Immunohistochemistry of paraffin-embedded human brain tissue using PACO41818 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human endometrial cancer using PACO41818 at dilution of 1:100.
Background:
Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase (By similarity).
RABGEF1: Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase. Interacts with RGS14; the interaction is GTP-dependent. Heterodimer with RABEP1. The heterodimer binds RAB4A and RAB5A that have been activated by GTP-binding. Interacts with RAB21, and with 100-fold lower affinity also with RAB22. Binds TSC2, GGA1, GGA2, GGA3, AP1G1 and AP1G2. Interacts with ubiquitinated EGFR. 3 isoforms of the human protein are produced by alternative splicing.Protein type: GAP, Rab; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 7q11.21Cellular Component: early endosomeMolecular Function: protein binding; Rab GTPase binding; Rab guanyl-nucleotide exchange factor activityBiological Process: protein targeting to membrane
UniProt Protein Details:
NCBI Summary:
RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010]
