The Rabbit Anti-His Tag Polyclonal Antibody (CABE068) is a valuable tool for researchers working with His-tagged proteins in various applications. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting His-tagged proteins in Western blot assays. The His tag, commonly added to recombinant proteins for purification and detection purposes, can be easily recognized by this antibody, allowing for efficient and accurate analysis.With its strong reactivity towards His-tagged proteins, this antibody is ideal for use in protein expression studies, protein purification processes, and protein-protein interaction studies.
Its versatility and reliability make it a must-have reagent for scientists working in disciplines such as molecular biology, biochemistry, and biotechnology.In conclusion, the Rabbit Anti-His Tag Polyclonal Antibody (CABE068) offers researchers a valuable tool for detecting and analyzing His-tagged proteins with ease and precision. Its high reactivity and specificity make it a reliable choice for a wide range of research applications, making it a key component in the toolkit of any scientist working with His-tagged proteins.
Product Name:
Rabbit anti His-Tag Polyclonal Antibody
SKU:
CABE068
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
A synthetic peptide corresponding to His tag.
Sequence:
HHHH HH
Tested Applications:
WBIF/ICCIP
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
His;His tag;His-tag
Positive Sample:
293T
Observed MW:
35kDa
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (AE068) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
