The PUS10 Polyclonal Antibody (CAB15962) is a valuable tool for researchers studying PUS10, a protein involved in RNA modification and processing. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. It specifically binds to the PUS10 protein, allowing for precise detection and analysis in a variety of cell types.PUS10 plays a crucial role in modifying RNA molecules, particularly tRNAs, to ensure their proper function in protein synthesis.
Dysregulation of PUS10 has been implicated in various diseases, including cancer and neurological disorders. By studying PUS10 with this antibody, researchers can gain insight into its function and potential therapeutic applications in these conditions.Overall, the PUS10 Polyclonal Antibody is an essential tool for investigating the role of PUS10 in RNA biology and disease pathogenesis, making it a valuable asset for research in molecular biology and biomedicine.
Product Name:
PUS10 Rabbit Polyclonal Antibody
SKU:
CAB15962
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2).
Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).
Purification Method:
Affinity purification
Gene ID:
150962
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PUS10 Rabbit pAb (CAB15962) at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
