The Puromycin Polyclonal Antibody (CAB21205) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with puromycin, allowing for precise detection and analysis in various experimental techniques such as immunohistochemistry, immunofluorescence, and flow cytometry.Puromycin is commonly employed in cell culture studies to selectively eliminate cells that are not incorporating the antibiotic into their protein synthesis machinery. The Puromycin Polyclonal Antibody provides researchers with a reliable means to monitor and assess the effectiveness of puromycin treatment in their experiments, enabling a better understanding of protein synthesis processes and cell viability.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Puromycin Polyclonal Antibody
SKU:
CAB21205
Size:
20μL, 100μL
Reactivity:
Species independent
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to Puromycin.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
293F, RAW264.7
Observed MW:
10-250kDa
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor.More recently, in vivo puromycylation assays have become popular tools for localizing translating ribosomes in cells. These assays often use elongation inhibitors to purportedly inhibit the release of puromycin-labeled nascent peptides from ribosomes.
Purification Method
Affinity purification
Gene ID
CAS:58-58-2
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293F cells, using Puromycin Rabbit pAb (CAB21205) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from RAW264.7 cells, using Puromycin Rabbit pAb (CAB21205) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
