The Puromycin Polyclonal Antibody is a valuable tool for researchers studying puromycin, a potent antibiotic that is widely used in biomedical research for protein synthesis inhibition. This antibody, produced in rabbits, is highly specific and reactive with puromycin, allowing for precise detection and analysis in various experimental techniques such as immunohistochemistry, immunofluorescence, and flow cytometry.Puromycin is commonly employed in cell culture studies to selectively eliminate cells that are not incorporating the antibiotic into their protein synthesis machinery. The Puromycin Polyclonal Antibody provides researchers with a reliable means to monitor and assess the effectiveness of puromycin treatment in their experiments, enabling a better understanding of protein synthesis processes and cell viability.
With its proven specificity and versatility, the Puromycin Polyclonal Antibody is an essential tool for researchers investigating protein synthesis, cell biology, and drug discovery. Its application in a wide range of research areas, including cancer biology, neuroscience, and microbiology, makes it a valuable asset for scientists seeking to further their understanding of puromycin's mechanisms of action and potential therapeutic applications.
Product Name:
Puromycin Polyclonal Antibody
SKU:
CAB21205
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Chemical compounds corresponding to Puromycin.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Positive Sample:
293F,RAW264.7
Conjugate:
Unconjugated
Observed MW:
10-250kDa
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor.More recently, in vivo puromycylation assays have become popular tools for localizing translating ribosomes in cells. These assays often use elongation inhibitors to purportedly inhibit the release of puromycin-labeled nascent peptides from ribosomes.
Purification Method:
Affinity purification
Gene ID:
CAS:58-58-2
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293F cells, using Puromycin Rabbit pAb (CAB21205) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
