PSME1 Rabbit Polyclonal Antibody (CAB5358)
- SKU:
- CAB5358
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | PSME1 Rabbit Polyclonal Antibody |
SKU: | CAB5358 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human PSME1 (NP_006254.1). |
Sequence: | MAML RVQP EAQA KVDV FRED LCTK TENL LGSY FPKK ISEL DAFL KEPA LNEA NLSN LKAP LDIP VPDP VKEK EKEE RKKQ QEKE DKDE KKKG EDED KGPP CGPV NCNE KIVV LLQR LKPE IKDV IEQL NLVT TWLQ LQIP RIED GNNF GVAV QEKV FELM TSLH TKLE GFHT QISK YFSE RGDA VTKA AKQP HVGD YRQL VHEL DEAE YRDI RLMV MEIR NAYA VLYD IILK NFEK LKKP RGET KGMI Y |
Tested Applications: | WB IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 |
Synonyms: | PA28A; IFI5111; REGalpha; PA28alpha; HEL-S-129m; PSME1 |
Positive Sample: | MCF7,22Rv1,BT-474,Mouse spleen,Mouse liver,Mouse lung,Mouse heart,Mouse thymus |
Conjugate: | Unconjugated |
Cellular Localization: | cytoplasm, cytosol, extracellular exosome, nucleoplasm |
Calculated MW: | 29kDa |
Observed MW: | 31kDa |
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.
Purification Method: | Affinity purification |
Gene ID: | 5720 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |