The PSME1 Polyclonal Antibody (CAB5358) is a valuable tool for researchers studying proteasome activator complex subunit 1 (PSME1), a protein involved in the regulation of the proteasome pathway. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples.PSME1 is essential for the activation of the 20S core proteasome and plays a crucial role in protein degradation, cell cycle regulation, and antigen presentation. By targeting PSME1, researchers can gain insights into the mechanisms of proteasome function and potentially identify novel therapeutic targets for diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
With its high specificity and sensitivity, the PSME1 Polyclonal Antibody is an excellent tool for investigating the role of PSME1 in various biological processes and disease states. Its versatility and reliability make it an essential component of any research program focused on understanding the complex interplay of the proteasome pathway in health and disease.
Product Name:
PSME1 Rabbit Polyclonal Antibody
SKU:
CAB5358
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human PSME1 (NP_006254.1).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5720
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSME1 antibody (CAB5358) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
