The PSMB10 Monoclonal Antibody is a specialized tool for researchers studying the proteasome subunit beta type-10 (PSMB10) protein, which plays a vital role in cellular processes such as protein degradation and antigen presentation. This antibody, developed using hybridoma technology, offers high specificity and sensitivity in detecting PSMB10 in various experimental settings, including Western blot and immunohistochemistry.PSMB10 is a key component of the immunoproteasome, an alternative form of the proteasome involved in generating peptides for major histocompatibility complex (MHC) class I antigen presentation.
Dysregulation of PSMB10 expression has been linked to various diseases, including cancer and autoimmune disorders. By using the PSMB10 Monoclonal Antibody, researchers can gain valuable insights into the function and regulation of PSMB10, paving the way for potential therapeutic interventions targeting the immunoproteasome in disease settings.
Product Name:
PSMB10 Monoclonal Antibody
SKU:
CAB21123
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (P40306).
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
Purification Method:
Affinity purification
Gene ID:
5699
Clone Number:
ARC2999
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSMB10 antibody (CAB21123) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
