The PRIM2 Polyclonal Antibody (CAB11633) is a vital tool for researchers studying PRIM2, a key enzyme involved in DNA replication and repair processes. This antibody, produced through rabbit immunization, exhibits high specificity and reactivity towards human samples, making it a reliable option for a variety of experimental applications. Validated for use in Western blotting, the PRIM2 antibody enables the precise detection and analysis of PRIM2 protein levels in different cell types, facilitating investigations in molecular biology and cancer research.PRIM2, also referred to as DNA primase subunit 2, plays a crucial role in the initiation of DNA synthesis by synthesizing short RNA primers that are essential for DNA polymerase to elongate DNA strands during replication.
Dysregulation of PRIM2 expression or activity can lead to genomic instability and contribute to the development and progression of various diseases, including cancer. Therefore, exploring the function and regulation of PRIM2 is crucial for advancing our understanding of DNA replication mechanisms and identifying potential therapeutic targets for cancer treatment.
Product Name:
PRIM2 Rabbit Polyclonal Antibody
SKU:
CAB11633
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_000938.2).
This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
5558
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRIM2 Rabbit pAb (CAB11633) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
