PP2A-Aβ/PR65β/PPP2R1B Polyclonal Antibody (CAB21559)
- SKU:
- CAB21559
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IF
Description
| Product Name: | PP2A-Aβ/PR65β/PPP2R1B Polyclonal Antibody |
| SKU: | CAB21559 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 468-667 of human PP2A-Aβ/PR65β/PPP2R1B (NP_859050.1). |
| Sequence: | YAIR EAAT NNLM KLVQ KFGT EWAQ NTIV PKVL VMAN DPNY LHRM TTLF CINA LSEA CGQE ITTK QMLP IVLK MAGD QVAN VRFN VAKS LQKI GPIL DTNA LQGE VKPV LQKL GQDE DMDV KYFA QEAI SVVA QRLR KLEF PVKD SGEP SVPR ADKN HFPR PTVP GEDM GKGP VYQL RGDT RDTL AQLG IAEL VHFS QSTD |
| Tested Applications: | WB IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 |
| Synonyms: | PR65B; PP2A-Abeta; 1B |
| Positive Sample: | hek293T |
| Conjugate: | Unconjugated |
| Cellular Localization: | cytoplasm, extracellular exosome |
| Calculated MW: | 66kDa |
| Observed MW: | 66kDa |
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described.
| Purification Method: | Affinity purification |
| Gene ID: | 5519 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of extracts from wild type(WT) and PP2A-Aβ/PR65β/PPP2R1B knockout (KO) hek293T(KO) cells, using PP2A-Aβ/PR65β/PPP2R1B antibody (CAB21559) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s. |
Related Products
